Optımızatıon of Condıtıons for the Productıon of Indole Acetıc Acıd by Azotobacter spp.

Azotobacter spp. are known for their ability to fix nitrogen into the soil non-symbiotically. Their activities can be enhanced through provision of optimum cultural conditions. Hence, this study aimed isolate and optimize growth (medium condtions) with a focus on pH, sucrose indole acetic acid (IAA) concentrations. The counts obtained from rhizosphere crops ranged 4.0 × 104 – 1.0 106 CFU/g. three high IAA-producing were identified as A. chroococcum, vinelandii beijerinckii. They produced IAA in absence presence 0.25 % tryptophan ranges 0.20 0.36 604.5 1439.7 µg/mL respectively. However, under optimized conditions these isolates folds. Optimum was by beijerinckii at concentration ranging 6.5 7.5, 2 3 0.3 0.7 %. higher amount when compared chroococcum optimal These 2001.1, 2541.1 2602.6 pH (2 %) (0.3%) It concluded findings that, is an excellent producer plant promoting hormone, indole-3-acetic (IAA).